Journal: PLoS ONE

Article Title: Angiotensin-(1-7) Attenuates Kidney Injury Due to Obstructive Nephropathy in Rats

doi: 10.1371/journal.pone.0142664

Figure Lengend Snippet: (a) NRK-52E cells were exposed to Ang II (1 μM, 16 h) with or without 1 h pretreatment with Ang-(1–7) (1 μM) and Mas receptor antagonist A779 (1 μM). Each column represents mean ± SEM. * P < 0.05. ** P < 0.01. Data are representative of at least three independent experiments. (b) AT 1 R expression (red) was examined in NRK-52E cells after treatment with Ang II, Ang-(1–7), and A779; nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI) for immunofluorescence study. Original magnification, 400×. Scale bar = 50 μm. MasR, Mas receptor.

Article Snippet: The cells were then treated using Ang II (1 μM, H-1705; Bachem Americas, Torrance, CA) with or without 1 h of pretreatment with Ang-(1–7) (1 μM) and Mas receptor antagonist A779 (1 μM, H-2888; Bachem Americas, Torrance, CA).

Techniques: Expressing, Immunofluorescence

Journal: PLoS ONE

Article Title: Angiotensin-(1-7) Attenuates Kidney Injury Due to Obstructive Nephropathy in Rats

doi: 10.1371/journal.pone.0142664

Figure Lengend Snippet: NRK-52E cells were exposed to Ang II (1 μM, 16 h) with or without 1 h pretreatment with Ang-(1–7) (1 μM) and Mas receptor antagonist A779 (1 μM). (a) TGF-β1/Smad expression was analyzed. (b) The generation of reactive oxygen species (ROS) was detected using the ROS-sensitive fluorescent dye, DCF-DA. Each column represents the mean ± SEM. * P < 0.05. ** P < 0.01. Data are representative of at least three independent experiments.

Article Snippet: The cells were then treated using Ang II (1 μM, H-1705; Bachem Americas, Torrance, CA) with or without 1 h of pretreatment with Ang-(1–7) (1 μM) and Mas receptor antagonist A779 (1 μM, H-2888; Bachem Americas, Torrance, CA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Angiotensin-(1-7) Attenuates Kidney Injury Due to Obstructive Nephropathy in Rats

doi: 10.1371/journal.pone.0142664

Figure Lengend Snippet: NRK-52E cells were exposed to Ang II (1 μM, 16 h) with or without 1 h pretreatment with Ang-(1–7) (1 μM) and Mas receptor antagonist A779 (1 μM). (a) α-smooth muscle actin (α-SMA), connective tissue growth factor (CTGF), and fibronectin, as well as (b) the Bax and Bcl-2 protein expressions were then analyzed. (c) Downregulation of Mas receptor protein in NRK-52E cells at 48 h after Mas receptor siRNA transfection. NRK-52E cells were transfected with Mas receptor siRNA and then exposed to Ang II (1 μM, 16 h) with or without 1 h pretreatment with Ang-(1–7) (1 μM). Western blot analyses represented the (d) α-SMA, CTGF and fibronectin, as well as (e) the Bax and Bcl-2 protein expression. Each column represents the mean ± SEM. * P < 0.05. ** P < 0.01. Data are representative of at least three independent experiments.

Article Snippet: The cells were then treated using Ang II (1 μM, H-1705; Bachem Americas, Torrance, CA) with or without 1 h of pretreatment with Ang-(1–7) (1 μM) and Mas receptor antagonist A779 (1 μM, H-2888; Bachem Americas, Torrance, CA).

Techniques: Transfection, Western Blot, Expressing

Journal: PLoS ONE

Article Title: Angiotensin-(1-7) Attenuates Kidney Injury Due to Obstructive Nephropathy in Rats

doi: 10.1371/journal.pone.0142664

Figure Lengend Snippet: (a) Ang II (1 μM, 16 h) with or without 1 h pretreatment with Ang-(1–7) (1 μM) and Mas receptor antagonist A779 (1 μM). Each column represents the mean ± SEM. * P < 0.05. Data are representative of at least three independent experiments. (b) Phosphorylated histone H3 (Ser 10) expression (red) was examined in NRK-52E cells after treatment with Ang II, Ang-(1–7), and A779; nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI) for immunofluorescence study. Original magnification, 400×. Scale bar = 20 μm.

Article Snippet: The cells were then treated using Ang II (1 μM, H-1705; Bachem Americas, Torrance, CA) with or without 1 h of pretreatment with Ang-(1–7) (1 μM) and Mas receptor antagonist A779 (1 μM, H-2888; Bachem Americas, Torrance, CA).

Techniques: Expressing, Immunofluorescence

Journal: PLoS ONE

Article Title: Angiotensin-(1-7) Attenuates Kidney Injury Due to Obstructive Nephropathy in Rats

doi: 10.1371/journal.pone.0142664

Figure Lengend Snippet: NRK-52E cells were exposed to Ang II (1 μM, 16 h) with or without 1 h pretreatment with Ang-(1–7) (1 μM) and Mas receptor antagonist A779 (1 μM). Each column represents the mean ± SEM. * P < 0.05. ** P < 0.01. Data are representative of at least three independent experiments.

Article Snippet: The cells were then treated using Ang II (1 μM, H-1705; Bachem Americas, Torrance, CA) with or without 1 h of pretreatment with Ang-(1–7) (1 μM) and Mas receptor antagonist A779 (1 μM, H-2888; Bachem Americas, Torrance, CA).

Techniques: